REQUIREMENTS
| Laboratory Analysis/ Diagnosis | Samples to be submitted | Requirement |
| 1. PARASITOLOGY
a. Blood Parasite Examination (Surra, Anaplasma, Babesia) b. Fecalysis b.1 Floatation Technique b.2 Sedimentation b.3 Larval Culture b.4 Direct Smear c. Direct Microscopy (External Parasite Identification) |
Whole blood (test tube with edta)
Feces Feces Feces Feces Feces Skin scraping |
Fresh, atleast 1 mL., transport with ice
Fresh 5-10 grams/sample, 6 hours after collection, transport with ice
Fresh, in glass slide or place in a clean/sterile tube |
| 2. PATHOLOGY
a. Clinical Pathology a.1 Complete Blood Count a.2 Blood Chemistry Analysis b. Histopathology
c. Necropsy |
Whole blood Serum Tissue/Organ, i inch by 1 inch from the affeted organ or where the significant lession is found Live/ Dead Animal
|
Fresh, 12 hours after collection Fresh, 12 hours after collection Preserved in 10% formalin
Within 6 hours after death
|
| 3. MICROBIOLOGY
a. Bacterial Culture b. Bacterial Isolation and Identification c. Antibiotic Sensitivity Test d. Total Bacterial Count for milk and water e. Fungal Isolation and Identification f. Heterotrophic Plate Count |
Tissue/ Organ Tissue/ Organ Tissue/ Organ
water/ milk
Tissue/ Organ Water |
Fresh, usually taken from necropsied animals Fresh Fresh Fresh, place in clean/sterile container
Fresh Fresh
|
| 4. SEROLOGY
a. ELISA (AI, FMD, Brucella, Anaplasma, Babesia, CAE, NCD) b. Card Agglutination Test for Surra c. Agar Gel Immuni Diffusion Test for Equine Infectious Anemia and Blue Tongue d. Hemagglutination Inhibition Test for Avian Influenza and Newcastle Disease
|
Serum
Serum
Serum
Serum
|
Fresh, 12 hours after collection
Fresh, 12 hours after collection
Fresh, 12 hours after collection
Fresh, 12 hours after collection
|
| 5. VIROLOGY
a. Rabies |
Dog Head |
Fresh, within 6 hours after death, transport with ice |
| 6. Polymerase Chain Reaction Test
a. Hemorrhagic Septicemia b. ORF (Contagious Ecthyma) c. Blood Parasites ( Babesia, Anaplasma, Surra 0r Trypanosoma) |
Whole blood
Whole blood Whole blood |
Fresh, atleast 1mL |
FEES
INTRODUCTION & GENERAL INFORMATION:
The Cagayan Valley Integrated Agricultural Laboratory (CVIAL) is envisioned to be the center of sterling and globally competitive laboratory services for food safety, increased productivity and agripreneurship, with the mandate of harmonizing provision of laboratory services and products that ensure consumer and environment safety, productivity of soil, crops, and livestock, increased income and improved quality of life of farmers, fishermen, and other stakeholders.
The main function of CVIAL is to provide a modernized and comprehensive agri-laboratory services for quality, food safety, and increased productivity amidst Climate Change. The impacts of CVIAL’s services are increased production, high quality products, ensured bio-safety and bio-security and increased product consumer’s acceptability. Strengthening of the CVIAL’s capabilities is therefore very essential to respond to the challenges of the time. This also means the strengthening and enforcement of the collection of fees for services rendered to all clients. We have been mandated by the Central Office ever since to collect fees for laboratory tests and legal basis are available. Hence, all the four laboratories under CVIAL are to collect fees starting July 1, 2018.
PROCEDURES:
- Clients will be classified according to:
- Students conducting undergraduate thesis – 50% discount.
- Masteral students will pay in full.
- Indigent farmers will not pay as long as they will present a certification of indigency duly signed by their barangay officials. They should present this document upon receipt of the laboratory result of their samples.
- Animal Raisers/Ranchers/Farm owners will pay in full, however they have the option to provide their test kits for requested tests and will not pay.
- Students and other clients requesting for visits and conduct of their thesis shall submit a request letter addressed to the Regional Executive Director attention to the Integrated Laboratory Division Chief. The letter should be duly signed by their Dean and adviser and submit 2 weeks before the conduct of their thesis or visits. They shall also fill up the Facility Access Form one week prior to the conduct of their requests. (see attached)
- Students requesting for the conduct of their undergraduate or masteral thesis shall be required to do their laboratory work and not the laboratory staff to perform for them. The laboratory staff will only supervise.
- They are required to bring their own laboratory gowns and other things needed in the conduct of their thesis and shall replace any glass wares and other laboratory materials that they will break or destroy.
- They shall follow all the Good Laboratory Practices required in the lab. (no cell phones, no drinks and food, no application of cosmetics, etc.) – see attached.
- Students who conducted their thesis shall give a file copy of their manuscript to CVIAL.
REGIONAL ANIMAL DISEASE DIAGNOSTIC LABORATORY: (FROM BAI)
Pursuant to Executive Order No. 197 issued by the Office of the President dated June 13, 2000, AO # 25, series 2003, mandating the increase in fees and services by not less than 20%, the following schedule of fees and charges for diagnostic services rendered by the Animal Disease Diagnostic Reference Laboratory (formerly Philippine Animal Health Center), Bureau of Animal Industry, is hereby promulgated for the information and guidance of all Regional Animal Disease Diagnostic Laboratories in the Philippines.
| I. PATHOLOGY | DIAGNOSTIC TESTS | LABORATORY FEES (Proposed) |
| A. | Gross Pathology (Necropsy)a | |
| 1. | Poultry/Pet Birds – Up to 2 months old | 75.00/head |
| 2. | Poultry/Pet Birds – Over 2 months old | 100.00/head |
| 3. | Pig – Up to 2 months old | 150.00/head |
| 4. | Pig – Over 2 months old | 200.00/head |
| 5. | Pig – Sow/Boar | 300.00/head |
| 6. | Goat/Sheep | 300.00/head |
| 7. | Dog – Small Breed | 200.00/head |
| 8. | Dog – Large Breed | 300.00/head |
| 9. | Cat/Other small animals | 200.00/head |
| 10. | Cattle/Carabao/Horse – Up to 2 months old | 300.00/head |
| 11. | Cattle/Carabao/Horse – Over 2 months old | 500.00/head |
| B. | Histopathologyb | |
| 1. | Tissue processing (without reading): | |
| a) | Up to 10 slides | 500.00/case |
| b) | Over 10 slides | 50.00/additional slide |
| 2. | ||
| a) | Up to 10 slides | 1,000.00/case |
| b) | Over 10 slides | 100.00/additional slide |
| 3. | ||
| a) | Up to 10 slides | 500.00/case |
| b) | Over 10 slides | 50.00/additional slide |
| C. | Clinical Pathologyb | |
| 1. | Complete Blood Count (CBC) includes: | 300.00/blood sample |
| a) | RBC Count | 100.00/blood sample |
| b) | WBC Count | 100.00/blood sample |
| c) | Differential Count | 100.00/blood sample |
| d) | Hemoglobin | 100.00/blood sample |
| e) | Hematocrit | 100.00/blood sample |
| D. | Immunohistochemistryb | 400.00/sample/virus |
| II. VIROLOGY | ||
| A. Viral Isolation & Identification | ||
| 1. | Tissue Culture | 900.00/sample/virus |
| 2. | Egg Inoculation | 450.00/sample |
| B. Serology | ||
| 1. | Agar Gel Immunodiffusion (AGID)c: | |
| Ruminant: | ||
| a) | Bluetongue (BT) | 300.00/serum sample |
| b) | Caprine Arthritis-Encephalitis (CAE) | 300.00/serum sample |
| Poultry: | ||
| a) | Avian Encephalomyelitis (AE) | 300.00/serum sample |
| b) | Viral Arthritis/Avian Reovirus | 300.00/serum sample |
| c) | Gumboro/Infectious Bursal Disease (IBD) | 300.00/serum sample |
| d) | Infectious Laryngotracheitis (ILT) | 300.00/serum sample |
| e) | Marek’s Disease (MD) | 300.00/serum sample |
| f) | Avian Influenza (AI) | 300.00/serum sample |
| Equine: | ||
| a) | Coggins’ Test/Equine Infectious Anemia (EIA) | 250.00/serum sample |
| 2. | Enzyme-Linked Immunosorbent Assay (ELISA)d: | |
| All ELISAs not listed below | 350.00/serum sample | |
| Ruminant: | ||
| a) | Foot-and-Mouth Disease (FMD) | |
| i. | Liquid Phase Blocking ELISA (LPBE) | 500.00/serotype/serum sample |
| ii. | Non-Structural Protein ELISA (NSPE) | No charge |
| iii. | FMD Typing ELISA | No charge |
| b) | Caprine Arthritis-Encephalitis (CAE) | 350.00/serum sample |
| c) | Bluetongue (BT) | 350.00/serum sample |
| d) | Bovine Leukosis (BL) | 350.00/serum sample |
| e) | Bovine Ephemeral Fever (BEF) | 350.00/serum sample |
| f) | Bovine Viral Diarrhea (BVD) | 350.00/serum sample |
| g) | Bovine Herpesvirus Infection | 350.00/serum sample |
| Pig: | ||
| a) | Aujeszky’s Disease or Pseudorabies (PRV) | 350.00/serum sample |
| b) | Foot and Mouth Disease (FMD) | |
| i. | Liquid Phase Blocking ELISA (LPBE) | 500.00/serotype/serum sample |
| ii. | Non-Structural Protein ELISA (NSPE) | No charge |
| iii. | FMD Typing ELISA | No charge |
| c) | Porcine Reproductive & Respiratory Syndrome (PRRS) | 400.00/serum sample |
| d) | Hog Cholera (HC) Antibody Detection ELISA | 350.00/serum sample |
| e) | Hog Cholera (HC) Antigen Detection ELISA | 400.00/sample |
| f) | Swine Influenza (SI) | 350.00/serotype/serum sample |
| g) | Porcine Circovirus 2 Associated Diseases (PCV2 AD) | 350.00/serum sample |
| h) | Porcine Epidemic Diarrhea (PED) | 350.00/serum sample |
| Poultry: | ||
| a) | Avian Reovirus/Viral Arthritis | 350.00/serum sample |
| b) | Avian Encephalomyelitis (AE) | 350.00/serum sample |
| c) | Chicken Anemia (CA) | 350.00/serum sample |
| d) | Infectious Bursal Disease (IBD) | 350.00/serum sample |
| e) | Infectious Laryngotracheitis (ILT) | 350.00/serum sample |
| f) | Newcastle Disease (ND) | 350.00/serum sample |
| g) | Swollen Head Syndrome (SHS) | 350.00/serum sample |
| h) | Avian Influenza (AI) | 350.00/serum sample |
| i) | Marek’s Disease (MD) | 350.00/serum sample |
| 3. | Hemagglutination (HA)/Hemagglutination-Inhibition (HI) Test: | |
| Poultry: | ||
| a) | Avian Adenovirus/Egg Drop Syndrome (EDS) | 100.00/serum sample |
| b) | Infectious Bronchitis (IB) | 100.00/serum sample |
| c) | Newcastle Disease (ND) | 75.00/serum sample |
| d) | Avian Influenza (AI) | 150.00/serotype/serum sample |
| Pig: | ||
| a) | Porcine Parvovirus (PPV) | 200.00/serum sample |
| b) | Swine Influenza (SI) | 150.00/serotype/serum sample |
| Horse: | ||
| a) | Equine Influenza | 150.00/serotype/serum sample |
| 4. | Virus/Serum Neutralization Test (VNT/SNT): | |
| Pig: | ||
| a) | Hog Cholera (HC) | 500.00/serum sample |
| b) | Aujeszky’s Disease or Pseudorabies (PRV) | 500.00/serum sample |
| C. | Immunofluorescence Assay (IFA)b, f | |
| Pig: | ||
| a) | Aujeszky’s Disease or Pseudorabies (PRV) | 400.00/sample |
| b) | Hog Cholera (HC) | 400.00/sample |
| c) | Porcine Reproductive and Respiratory Syndrome (PRRS) | 400.00/sample |
| d) | Porcine Circovirus 2 Associated Disease (PCV2 AD) | 400.00/sample |
| e) | Swine Influenza (SI) | 400.00/sample |
| f) | Transmissible Gastroenteritis (TGE) | 400.00/sample |
| g) | Porcine Epidemic Diarrhea (PED) | 400.00/sample |
| h) | Rotaviral Infection | 400.00/sample |
| Poultry: | ||
| a) | Chicken Anemia (CA) | 400.00/sample |
| b) | Infectious Laryngotracheitis (ILT) | 400.00/sample |
| D. | Rabies Examination | |
| 1. | Direct Microscopic Examination (DME) | 180.00/sample |
| 2. | Fluorescent Antibody Test (FAT) | 200.00/sample |
| 3. | Mouse Inoculation Test (MIT) | 200.00/sample |
| III. BACTERIOLOGY | ||
| A. | General Bacterial Isolation & Identification | 300.00/organ or sample |
| B. | Anaerobic Isolation & Identification | 500.00/organ or sample |
| C. | Salmonella Isolation | 720.00/sample |
| D. | Antibiotic Sensitivity Test (AST) | 300.00/sample |
| E. | Fungal Isolation & Identification | 300.00/sample |
| F. | Bacterial Count | 600.00/sample |
| G. | Fungal Count | 600.00/sample |
| H. | Water Coliform Count | 350.00/sample |
| I. | Serology: | |
| 1. | Rose Bengal Precipitation Test (RBPT) | |
| Cattle/Pig/Goat/Sheep/Dog: | ||
| a) | Brucella spp. | 200.00/sample |
| b) | Leptospira spp. | 200.00/sample |
| 2. | Rapid Plate Agglutination Test (RPAT) | |
| Poultry: | ||
| a) | Mycoplasma gallisepticum | 200.00/sample |
| b) | Mycoplasma synoviae | 200.00/sample |
| c) | Salmonella pullorum | 200.00/sample |
| 3. | Tube Agglutination Test: | |
| Cattle/Pig/Goat/Sheep/Dog: | ||
| a) | Brucella spp. | 350.00/sample |
| 4. | Slide Agglutination Test: | |
| a) | Brucella Monoclonal Antibody Agglutination Test | |
| (A, M, R antiserum) | 350.00/sample | |
| b) | Salmonella Enteridis Antiserum | 350.00/sample |
| 5. | Enzyme-Linked Immunosorbent Assay (ELISA)b,d: | |
| a) | Brucella spp. | 350.00/sample |
| b) | Clostridium Perfringes Enterotoxaemia | 350.00/sample |
| c) | Mycoplasma hyopneumoniae | 350.00/sample |
| d) | Actinobacillus Pleuropneumoniae | 350.00/sample |
| e) | Hemophilis sp. | 350.00/sample |
| f) | Mycobacterium sp. | 350.00/sample |
| g) | Salmonella sp. | 350.00/sample |
| h) | Pasturella sp. | 350.00/sample |
| 6. | Complement Fixation Test | |
| IV. PARASITOLOGY | ||
| A. | Blood Parasite Examination (BPE)f | |
| 1. | Direct Smear | 30.00/sample |
| 2. | Stained Smear | 50.00/sample |
| 3. | Hematocrit Centrifuge Technique with Packed Cell Volume | 50.00/sample |
| 4. | Mouse Inoculation Test | 200.00/sample |
| B. | Fecal Examination (Fecalysis)f | |
| 1. | Qualitative: | |
| a) | Direct Smear | 30.00/sample |
| b) | Simple Flotation | 50.00/sample |
| 2. | Quantitative: | |
| a) | McMaster Counting Technique (Egg Per Gram Counting) | 50.00/sample |
| b) | Sedimentation Technique (for trematodes) | 50.00/sample |
| c) | Larval Culture | 200.00/sample |
| C. | Serologyb | |
| 1. | Card Agglutination Test | |
| Horse/Cattle/Buffalo: | ||
| a) | Card Agglutination Test for Trypanosomes (CATT) | 200.00/sample |
| 2. | Enzyme-Linked Immunosorbent Assay (ELISA) | |
| Cattle/Buffalo: | ||
| a) | Anaplasma marginale | 350.00/sample |
| Pig: | ||
| a) | Trichinella spiralis | 400.00/sample |
| b) | Toxoplasma gondii | 400.00/sample |
| D. | Identification of Parasite: | |
| 1. | Tissue Parasite | 200.00/parasite sample |
| 2. | Whole Parasite (non-tissue) | 100.00/parasite sample |
| 3. | Skin Scraping | 50.00/sample |
| V. MOLECULAR TECHNIQUESb,f | ||
| A. | Conventional PCR (single agent) | 2,500.00/sample |
| B. | Real-Time PCR – qPCR (single agent) | 3,000.00/sample |
| C. | Multiplex qPCR/PCR | 3,500.00/sample |
| D. | Serotyping PCR | 4,000.00/sample |
| E. | Insulated Isothermal PCR (iiPCR) | 1,500.00/sample |
| F. | Loop-Mediated Isothermal Amplification (LAMP) | 500.00/sample |
| VI. TOXICOLOGY | ||
| A. | Aflatoxin Analysisg | 1,300.00/sample |
| B. | Analysis of other Mycotoxinsg | 1,300.00/sample |
| C. | Toxicological Examination: | |
| 1. | Volatile | 450.00/sample |
| 2. | Non-volatile | 450.00/sample |
| D. | Toxicity Test | 300.00/sample |
| VII. OTHERS | ||
| A. | Diagnostic Laboratory Accreditation | |
| Application Fee | 300.00 | |
| Accreditation Fee | 3,000.00 | |
| Renewal Fee | 2,000.00 | |
| B. | Certified True Copy of Laboratory Results/Documents | 25.00/page |
| aReceiving of samples up to 3:00 PM, Monday to Friday | |
| bDepending upon the availability of reagents/kits/antigen/antiserum | |
| cRequires 3 or 4 samples to run the test | |
| dTest will be run only if the no. of samples is enough to fill an ELISA plate | |
| eSubmission of samples (for farm/animal consumption only) Monday to Wednesday only | |
| fDuration of test depends on the no. of samples/cases | |
| gRequires at least 5 samples to run the test | |
STEPS
REGIONAL ANIMAL DISEASE DIAGNOSTIC LABORATORY (RADDL)
| STEPS | PROCESS | AGENCY ACTION | DURATION OF ACTIVITY | PERSON-IN-CHARGE |
| 1 | Proceed to the Information Desk/ Receiving Area | Lab. Personnel will record necessary information on the logbook | 10 mins | Laboratory Aide |
| Inspection of samples | Lab. Personnel will inspect if the samples are fit for analysis.
If YES, samples will be sent to the laboratory for analysis. If NO, non-conforming samples are handled accordingly. |
5 mins | Laboratory Aide | |
| 2 | For Analysis: Fill-up and submit request forms | Lab Personnel will issue claim stub to client and assign Laboratory number. | 5 mins | Laboratory Aide |
| For Laboratory Products: Fill-up Requisition and Issue Slip (RIS) | Prepare laboratory product requested | 15 mins | Laboratory Aide | |
| 3 | Send sample for analysis | Samples will be sent to the concerned laboratory for analysis | 5 mins | Laboratory Aide |
| 4 | Conduct Analysis | Samples will be analyzed as per request of client | 7 days | Laboratory Analyst |
| 5 | Encoding of result, interpretation and recommendation. | Results will be encoded in the | 7 days | Laboratory Analyst |
| 6 | Review and Approval of report of analysis and recommendation | Report of analysis and recommendation will be reviewed by Laboratory Chief, which will be released upon the approval of the Chief Agriculturist | 10 mins | Lab. Cgief/ Chief Agriculturist |
| 7 | Release of Report of Analysis to Clients | Original copy of the DA-RFO2-ILD-FORM 06 Report of Analysis will be release and claim by the client.
Client signs in the release logbook and fills up DA-RFO2-ILD-FORM 10 Client Satisfaction Feedback Form. |
10 mins
|
Laboratory Aide |